ISOLATION OF FUNGI FROM THE HANDS OF PRIMARY SCHOOL PUPILS

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PROJECT TOPIC AND MATERIAL ON ISOLATION OF FUNGI FROM THE HANDS OF PRIMARY SCHOOL PUPILS

The Project File Details

  • Name: ISOLATION OF FUNGI FROM THE HANDS OF PRIMARY SCHOOL PUPILS
  • Type: PDF and MS Word (DOC)
  • Size: [50 KB]
  • Length: [28] Pages

 

ABSTRACT

Children are predisposed to fungal skin infection and ill health from dirty hands which they use for their feeding and daily activities. This study focuses on isolating and identifying fungal organisms from the hands of primary school pupils and to determine the microbial load of the sample. The samples were collected under aseptic conditions and analyzed microbiologically. The streaking process was carried out on Sabouraud dextrose agar to isolate the fungal organisms present in the swab samples. The result of the direct plate count showed that the total number of isolates recorded was 33. Macroscopic and microscopic observation of the fungal organisms were done to identify morphological and structural appearance of the fungal growth and spores. Test such as germ tube test was done for further identification of yeasts. A total of 30 isolates of 4 genera were identified as follows; Candida, Aspergillus, Penicillium and Trichophyton. Aspergillius spp had the highest percentage occurrence of 40% while Candida spp had the lowest percentage occurrence of 15%. Washing of hands reduces the load of the fungal organisms, hence, I strongly recommend that hand washing facilities which include soap, disinfectant and clean water be provided for school children to maintain good hand washing practices as to reduce disease occurrence. Also the school management should ensure that children are taught how to wash their hands properly.

CHAPTER ONE

Title Page                                                                                                                                i

Certification                                                                                                                            ii

Approval Page                                                                                                                                    iii

Dedication                                                                                                                              iv

Acknowledgement                                                                                                                  v

Abstract                                                                                                                                  vi

Table of Content                                                                                                                     vii

List of Tables                                                                                                                          x

Introduction                                                                                                                            1

Literature Review                                                                                                                   4

Materials and Methods                                                                                                           14

Result                                                                                                                                      18

Discussion                                                                                                                               20

Conclusion and recommendation                                                                                           21

References                                                                                                                              22

Appendix                                                                                                                                23       

 

 

 

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